Chemists at MIT have adult a novel way to synthesize himastatin, a natural chemical compound that has shown potential as an antibiotic.

Using their new synthesis, the researchers were able not but to produce himastatin but also to generate variants of the molecule, some of which also showed antimicrobial activity. They also discovered that the chemical compound appears to kill bacteria by disrupting their cell membranes. The researchers now hope to pattern other molecules that could accept even stronger antibiotic activity.

“What we want to exercise right now is learn the molecular details well-nigh how it works, so we can design structural motifs that could better support that mechanism of activeness. A lot of our effort correct now is to learn more nigh the physicochemical backdrop of this molecule and how it interacts with the membrane,” says Mohammad Movassaghi, an MIT professor of chemical science and one of the senior authors of the written report.

Brad Pentelute, an MIT professor of chemistry, is also a senior author of the written report, which appears today in
Scientific discipline. MIT graduate student Kyan D’Angelo is the pb author of the study, and graduate student Carly Schissel is also an author.

Mimicking nature

Himastatin, which is produced past a species of soil bacteria, was offset discovered in the 1990s. In animal studies, it was found to accept anticancer activity, but the required doses had toxic side effects. The chemical compound likewise showed potential antimicrobial activity, but that potential hasn’t been explored in detail, Movassaghi says.

Himastatin, a naturally occurring compound with antibody properties, has an unusual homodimeric construction that makes it challenging to synthesize.

Himastatin is a complex molecule that consists of two identical subunits, known as monomers, that join together to class a dimer. The two subunits are hooked together past a bail that connect a 6-carbon band in i of the monomers to the identical band in the other monomer.

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This carbon-carbon bond is critical for the molecule’s antimicrobial activity. In previous efforts to synthesize himastatin, researchers have tried to make that bond first, using two uncomplicated subunits, and then added more circuitous chemic groups onto the monomers.

The MIT team took a dissimilar approach, inspired by the fashion this reaction is performed in bacteria that produce himastatin. Those bacteria have an enzyme that tin join the two monomers as the very terminal pace of the synthesis, by turning each of the carbon atoms that demand to exist joined together into highly reactive radicals.

To mimic that procedure, the researchers start congenital complex monomers from amino acrid building blocks, helped by a rapid peptide synthesis technology previously developed by Pentelute’s lab.

“By using solid-phase peptide synthesis, we could fast-forward through many synthetic steps and mix-and-match building blocks hands,” D’Angelo says. “That’due south just one of the means that our collaboration with the Pentelute Lab was very helpful.”

The researchers then used a new dimerization strategy developed in the Movassaghi lab to connect 2 complex molecules together. This new dimerization is based on the oxidation of aniline to form carbon radicals in each molecule. These radicals can react to grade the carbon-carbon bail that hooks the two monomers together. Using this approach, the researchers tin can create dimers that incorporate unlike types of subunits, in addition to naturally occurring himastatin dimers.

“The reason we got excited most this blazon of dimerization is because information technology allows you to really diversify the construction and admission other potential derivatives very quickly,” Movassaghi says.

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Membrane disruption

One of the variants that the researchers created has a fluorescent tag, which they used to visualize how himastatin interacts with bacterial cells. Using these fluorescent probes, the researchers institute that the drug accumulates in the bacterial cell membranes. This led them to hypothesize that information technology works by disrupting the jail cell membrane, which is also a mechanism used past at least one FDA-approved antibiotic, daptomycin.

The researchers too designed several other himastatin variants past swapping in different atoms in specific parts of the molecule, and tested their antimicrobial activity against six bacterial strains. They found that some of these compounds had potent activity, but merely if they included one naturally occurring monomer along with one that was unlike.

“By bringing 2 complete halves of the molecule together, we could make a himastatin derivative with only a unmarried fluorescent label. Only with this version could we do microscopy studies that offered evidence of himastatin’s localization within bacterial membranes, because symmetric versions with 2 labels did not have the right activity,” D’Angelo says.

Andrew Myers, a professor of chemistry at Harvard University, says that the new synthesis features “fascinating new chemical innovations.”

“This approach permits oxidative dimerization of fully synthetic monomer subunits to prepare the antibiotic himastatin, in a mode related to its biosynthesis,” says Myers, who was not involved in the research. “By synthesizing a number of analogs, of import construction-activity relationships were revealed, as well as bear witness that the natural product functions at the level of the bacterial envelope.”

The researchers now plan to pattern more than variants that they hope might have more than potent antibiotic action.

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“We’ve already identified positions that nosotros tin derivatize that could potentially either retain or enhance the activity. What’southward really exciting to us is that a significant number of the derivatives that nosotros accessed through this design process retain their antimicrobial activity,” Movassaghi says.

The research was funded by the National Institutes of Health, the Natural Sciences and Engineering Research Quango of Canada, and a National Science Foundation graduate enquiry fellowship.

Source: https://news.mit.edu/2022/himastatin-synthesis-chemical-0224